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intensity profile line tool  (Nikon)

 
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    Nikon intensity profile line tool
    Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence <t>intensity</t> measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity <t>profile</t> <t>line</t> <t>tool</t> in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.
    Intensity Profile Line Tool, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intensity profile line tool/product/Nikon
    Average 90 stars, based on 1 article reviews
    intensity profile line tool - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Ca 2+ -dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages * "

    Article Title: Ca 2+ -dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.743047

    Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence intensity measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity profile line tool in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.
    Figure Legend Snippet: Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence intensity measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity profile line tool in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.

    Techniques Used: Transfection, Incubation, Expressing, Confocal Microscopy, Fluorescence, Software



    Similar Products

    intensity profile line tool  (Nikon)
    90
    Nikon intensity profile line tool
    Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence <t>intensity</t> measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity <t>profile</t> <t>line</t> <t>tool</t> in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.
    Intensity Profile Line Tool, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intensity profile line tool/product/Nikon
    Average 90 stars, based on 1 article reviews
    intensity profile line tool - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    line intensity profile tool nis elements  (Nikon)
    90
    Nikon line intensity profile tool nis elements
    Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence <t>intensity</t> measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity <t>profile</t> <t>line</t> <t>tool</t> in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.
    Line Intensity Profile Tool Nis Elements, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/line intensity profile tool nis elements/product/Nikon
    Average 90 stars, based on 1 article reviews
    line intensity profile tool nis elements - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence intensity measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity profile line tool in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.

    Journal: The Journal of Biological Chemistry

    Article Title: Ca 2+ -dependent Focal Exocytosis of Golgi-derived Vesicles Helps Phagocytic Uptake in Macrophages *

    doi: 10.1074/jbc.M116.743047

    Figure Lengend Snippet: Role of voltage-gated Ca2+ channels in establishing the PIP3 gradient during phagocytosis. A, RAW264.7 macrophages were transfected with AKT-PH-mCherry. At 24 h, cells were incubated with GFP expressing E. coli for 5 min. In parallel, we also had AKT-PH-mCherry-expressing cells that were pretreated with EGTA (3 mm), loperamide (100 μm), amlodipine (100 μm), or wortmannin (5 μm) followed by incubation with GFP expressing E. coli for 5 min. Samples were fixed at 5 min and analyzed by confocal microscopy. The arrows in the far right image in each of the panels highlight the fluorescence intensity measurements for the analysis presented in B (scale bar, 4 μm). DIC, differential interference contrast. B, images in A (arrows) were analyzed using intensity profile line tool in the NIS elements software (see “Materials and Methods”). The data represent median from more than 20 fields for each case.

    Article Snippet: PIP 3 Gradient Analysis The gradient of fluorescence in the AKT-pH mCherry experiments was determined using “intensity profile line tool” in NIS-Elements (NIKON).

    Techniques: Transfection, Incubation, Expressing, Confocal Microscopy, Fluorescence, Software